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A level Biology B · Edexcel 9BI0 · Topic 5C

Photosynthesis

Key points

  • Chloroplasts have thylakoids stacked in grana (light-dependent reactions) surrounded by the stroma (light-independent reactions).
  • Light-dependent reactions: light excites electrons in chlorophyll in photosystems; photolysis of water replaces them, releasing O2_2; electron flow makes ATP (photophosphorylation) and reduced NADP.
  • Calvin cycle: RuBisCO fixes CO2_2 with RuBP to make two GP; GP is reduced to GALP using ATP and reduced NADP; most GALP regenerates RuBP, the rest makes glucose and other molecules.
  • Limiting factors: light intensity, CO2_2 concentration and temperature; the factor in shortest supply limits the rate.
  • Chromatography separates photosynthetic pigments; RfR_f = distance moved by pigment ÷ distance moved by solvent.
  • Accessory pigments absorb different wavelengths, widening the range of light used.

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Photosynthesis converts light energy into chemical energy stored in organic molecules. It happens in two linked stages in the chloroplast: the light-dependent reactions make ATP and reduced NADP, and the light-independent reactions use them…

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